■ 基本信息
別名: | pCDH-EF1a-MCS-T2A-copGFP |
啟動(dòng)子: | EF1α |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類: | 病毒系列，慢病毒克隆載體 |
質(zhì)粒大小: | 7266bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37℃ |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無須誘導(dǎo) |
5'測序引物:
| 根據(jù)序列設(shè)計(jì)引物
|
3'測序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,慢病毒 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | 基因CDS
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: | 綠色
|
■ 質(zhì)粒簡介
Stable overexpression with cDNA lentivectors Strong and ubiquitous expression of the gene of interestSingle or double expression cassette with choice of reporter geneTarget gene expressed from CMV, EF1, or MSCV promoter Choose from FIV- or HIV-based vectors Gene expression from constitutive promoters，The multiple cloning site (MCS) located downstream of an internal promoter allows convenient cloning of your gene of interest.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK782pCDH-EF1-MCS-T2A-copGFP慢病毒表達(dá)質(zhì)粒.txt
