■ 基本信息
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細(xì)胞，信號(hào)通路報(bào)告載體 |
質(zhì)粒大小: | 4079bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'測(cè)序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 信號(hào)報(bào)告 |
片段類(lèi)型: | Promoter
|
片段物種: |
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: | Fluc
|
■ 質(zhì)粒簡(jiǎn)介
The pRL-CMV Vector may be used in combination with any experimental reporter vector to co-transfect mammalian cells. However, it is important to realize that trans effects between promoters on co-transfected plasmids can potentially affect reporter gene expression . Primarily, this is of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements (such as CMV). The occurrence and magnitude of such effects will depend on several factors: a) the combination and activities of the genetic regulatory elements present on the co-transfected vectors, b) the relative ratio of experimental vector to control vector introduced into the cells, and c) the cell type transfected.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK489pRL-CMV哺乳報(bào)告質(zhì)粒.txt
